|
KMID : 0811720150190040327
|
|
Korean Journal of Physiology & Pharmacology
2015 Volume.19 No. 4 p.327 ~ p.334
|
|
|
Involvement of Heme Oxygenase-1 in Orexin-A-induced Angiogenesis in Vascular Endothelial Cells
|
|
Kim Mi-Kyoung
Park Hyun-Joo
Kim Su-Ryun
Choi Yoon-Kyung
Bae Soo-Kyung
Bae Moon-Kyoung
|
|
|
Abstract
|
|
|
|
The cytoprotective enzyme heme oxygenase-1 (HO-1) influences endothelial cell survival, proliferation, inflammatory response, and angiogenesis in response to various angiogenic stimuli. In this study, we investigate the involvement of HO-1 in the angiogenic activity of orexin-A. We showed that orexin-A stimulates expression and activity of HO-1 in human umbilical vein endothelial cells (HUVECs). Furthermore, we showed that inhibition of HO-1 by tin (Sn) protoporphryin-IX (SnPP) reduced orexin-A-induced angiogenesis in vivo and ex vivo. Orexin-A-stimulated endothelial tube formation and chemotactic activity were also blocked in SnPP-treated vascular endothelial cells. Orexin-A treatment increased the expression of nuclear factor erythroid-derived 2 related factor 2 (Nrf2), and antioxidant response element (ARE) luciferase activity, leading to induction of HO-1. Collectively, these findings indicate that HO-1 plays a role as an important mediator of orexin-A-induced angiogenesis, and provide new possibilities for therapeutic approaches in pathophysiological conditions associated with angiogenesis.
|
|
|
KEYWORD
|
|
|
Orexin-A, Heme oxygenase-1, Angiogenesis, Vascular endothelial cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|